Nested PCR procedure explained | Nested PCR for CSIR NET

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Nested PCR procedure explained | Nested PCR for CSIR NET - This lecture explains Nested PCR procedure explained | Nested PCR for CSIR NET.
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In 1st PCR set, why primer attach to unwanted DNA templates? As we design primer according to only our target DNA, then why primer attach to unwanted DNA flank side?

Jubokay
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Why can't we just go directly for the second set of reaction if that is more specific?

medhapathak
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দাদা এই ভিডিও টা তোমার অন্য ভিডিও র মতো ইনফরমেটিভ হলোনা, but খুব সুন্দর।।

devjotimondal
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After 1st round / primer adhere to target sequence. Is target sequence known ?

RVB
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Sir what will be the minimum mark to qualify csir net life sciences for general and ews

malinimalss
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