2019 novel coronavirus in culture

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Peter Doherty Institute for Infection and Immunity (Doherty Institute) scientists have successfully grown the 2019 novel coronavirus (COVID-19) from a patient sample, providing laboratories around the world with crucial information to help combat the virus.

This is a time-lapse video of a cell-culture infected with the novel coronavirus. A single layer of kidney cells is visible at the start of the footage and slowly, black dots appear across the cell sheet. The virus cannot be seen, but evidence of its presence can be as infected cells which appear as black dots. As more cells become infected with virus they lift off the cell layer and appear as black dots. More and more infected cells become visible. The video footage starts following 40hrs of culture and finishes following 80hrs of incubation.

Keep up to date with our work and news relating to 2019 novel coronavirus (COVID-19) here:

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Dear Doherty Institute since you have such had such a big hand in making out this is so different to other coronaviruses and influenzas we have had for decades please tell us that you have something more impressive by now. Something along the lines of 1. documentation proving ACTUAL ISOLATION of the virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and/or (SARS-CoV-2 Delta) which is confirmed by means of a purified viral sample being imaged with an electron microscope.
2. Documentation (a controlled scientific experiment in humans or animals) where the isolated SARS-CoV-2 virus (any strain) is exposed to healthy humans or animals and shown to cause the Coronavirus disease (COVID-19) with lungs filling with mucus.
Preferably this evidence should be provided according to Koch’s 4 postulates
(i) The microorganism must be found in diseased but not healthy
individuals;
(ii) The microorganism must be cultured from the diseased individual;
(iii) Inoculation of a healthy individual must recapitulate the disease;
(iv) The microorganism must be re-isolated and matched to the original
microorganism.”

Dream-Academy
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Fantastic job guys, thank you for your hard work and research!

bbglas
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Fascinating video, I've seen nothing like it. Thank you!

mariettabrill
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What cell line and in what media and conditions was this infection performed in?

SrirajaPanich
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Impressive ! Would it be possible to know a little bit more on the technical side of it : what cell line ? what imaging device (Iprasense's cytonote maybe) ? Thank you in advance

BernardCouvreur
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greetins from turkey great work everyone. humanity thanks you

franz
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how did you record this video?How much time is the actual video?

dr.msswapna
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How about a video of you zooming in on this slide from normal magnification?

kagrenac
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Can someone explain to me what any of this is? I'm not done with highschool bio yet, so all I'm seeing is some dots moving around on some white stuff. Are the dots the actual virii, or infected cells, or... lil grains of sand...? What's the white stuff? How sped up is this? How are the dots moving? What's special about these dots vs other kinds of dots?

adaritter
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Wow, , pantas aj.. Cepet bgt perkembangannya..

pencariilmu
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That are the cells affected by this virus right? The grow seems to be increasing extremely. Which imun system does that need..?

jonnygudman
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How did the chinese decode the corona virus genome and you have not done that, if you have not done that, what is being used to cross reference thhe thousands of tests taken from the publlic?

ChilliFedor
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Where can we find the publication, materials and methods?

alextrevelian
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Could you now investigate how long the Virus is able to live outside a human?

MaNu-tdzo
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Quite amazing but makes my skin crawl for some reason

PanclarksBrah
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Wait, are those black spots the virus? What kind of a microscope did you use to capture this footage?

hiran
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Hello! I was wondering if I can use this footage for a university project. I will credit the channel and the video used in the end credits! Thanks a lot in advance!

marinapaladi
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I’m confident with the cure being found

gta-gamer
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Someone tell me how the hell they know what this is, please?

MediaBuster
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Where are the viruses? They wouldn't be in this video because they're too small to see with a light microscope.

philipsmithnyc