Broth Microdilution assay - How to determine the MIC (Minimum Inhibitory Concentration)

Hey Scientists,

when working with antibiotics one needs to determine the MIC (=minimum inhibitory concentration) which is the lowest concentration that is sufficient to inhibit bacterial growth. The Broth Microdilution assay is the most commonly used assay to determine the compound concentration.

Cheers
Henrik

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References:

Time Codes:
0:00 Introduction
0:22 Antibiotics
0:55 MIC (Minimum Inhibitory Concentration)
1:43 Broth Microdilution assay
4:32 Outro

#Brothmicrodilution #MIC #antibiotics

[vinayashinde]

I have researcher interview tomorrow and this video helped me to clear the required basics, Thanksss🎉

[sofiera]

Your explanation is very clear, congrats!

[sanaamoha]

Thank you so much You explained it very well.🙏🏼

[Lanakalana]

That was such a clear explanation thankuuu

[ArnavPadhi]

Can u plz explain how different conc of the antibiotic solutions were made and their serial dilutions?
2. How much volume of bacteria was inoculated?
3. How much volume of the drug was added to the 96 well plate?

[nurulsuhana]

for the bacterial culture, how it can be changed from 1x10^8 cfu/ml to 5x10^5 cfu/ml when we need to transfer to 96 well plate ? can u explain the step

[youngscientistjomel]

May i l know what isbthe total volume in each well containing bacteria and drugs is it 100ul or 200 ul?

[moxiemania]

Corrections... The dilution of the drug is not serial* it's a doubling dilution

[olanma.o]

Thank you for this video ! Do you have one on MBC?

[yiwuzheng]

I also a little bit confuse about the selection of concentrations, why the mic is 4 rather than 3, or any value between 2-4

[arghayeaye]

Hello, at 4:20 there is a two fold decrease in concentrations and we concluded that the most diluted that prevents the growth is MIC. But how can we be sure that 4µg/ml is the MIC instead of say, 3µg/ml, a value in between 4 and 2 that cannot be observed by 2 fold decrease?

[asdbio]

Good evening, can we use this method to compare between antimicrobial effect of different solutions? Which wavelength should be used?

[eswinipi]

Is there any mathematical way to calculate MIC90 considering the results you got?

[Jaybee_]

what are the measurements when you use this method for fungi?

[noorfalak]

Plz share the link if disc diffusion method of microbial control of yours۔۔