Optimizing Transfection to Generate High Titer AAV and Lentiviral Vectors

Presented By: Sandy Tseng

Speaker Biography: Sandy joined the technical support team at Mirus Bio in 2019 and is responsible for working with scientists on troubleshooting and optimization of their gene delivery experiments and workflows. Before joining Mirus Bio, Sandy completed her doctoral thesis on the topic of regulation of eukaryotic gene expression at the University of Wisconsin-Madison.

Webinar: Optimizing Transfection to Generate High-Titer AAV and Lentiviral Vectors

Webinar Abstract: Recombinant adeno-associated virus (AAV) and lentiviral vectors (LV) are vehicles for direct delivery of therapeutic genes to patients' cells. In the coming years, the use of AAV and LV in clinical applications is projected to increase exponentially. Maximizing production of safe and effective viral vectors is essential to match this growing need. In this talk, we present optimization strategies for the transfection step of vector production to generate high titers of both AAV and LV using TransIT-VirusGEN® Transfection Reagent. The VirusGEN® AAV and LV Transfection Kits push the limits of high-titer virus production, which expand the manufacturing capabilities for gene and cell therapies.

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