What is FastQC Report?

Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. NGS has revolutionized the biological sciences, allowing labs to perform a wide variety of applications and study biological systems at a level never before possible.

A FASTQ file is a text file that contains the sequence data from the clusters that pass filters on a flow cell. In general, FASTA files are the most common standard for storing reference or consensus sequence data, while FASTQ is the most common format for storing raw sequence data. Raw reads are the fragments of DNA that are sequenced and stored in FASTQ file format.

FastQC is a tool that is used for the purpose of quality assessment of NGS data. Quality assessment identifies any potential problem or error in experimental setup and also it ensures that the correct samples have been sequenced with least amount of contamination and high amount of coverage. The overall quality of the sequence reads is assessed based on; Nucleotide distribution, Quality score distribution, GC-content, Amount of sequence ambiguity, Duplication of PCR(Polymerase chain reaction).

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