Synthetic Biology: Engineering bacteria with CRISPR - David Bikard

David Bikard’s talk focuses on engineering bacteria with CRISPR to combat microbial pathogens.  He explains how CRISPR technologies could eliminate antibiotic resistance.

Talk Overview:
Dr. David Bikard’s lab focuses on engineering bacteria with CRISPR to combat microbial pathogens. In this video, he introduces the historical context for using CRISPR in bacteria and then delves into two CRISPR technologies being developed by his lab. Part of his lab is using CRISPR/Cas9 to eliminate antibiotic resistance in bacterial populations. His group is also optimizing a catalytically dead Cas9 (dCas9) to modulate levels of CRISPR-induced transcriptional repression and use it in pooled high throughput screens for gene function.

Speaker Biography:
Dr. David Bikard is the head of the Synthetic Biology Group at the Institut Pasteur in Paris, France. Originally trained in engineering at AgroParisTech, he later received his Masters and PhD from Paris Diderot University for his work with the Institut Pasteur on the intergron bacterial recombination system. He began working with CRISPR during his postdoctoral fellowship with Dr. Luciano Marraffini at Rockefeller University. His work led to him becoming the founder and CSO of the company Eligo Biosciences. In 2014, he returned to the Institut Pasteur as an Investigator in Microbiology.

More information about David Bikard’s work can be found on his lab website:

[raptorrogue]

I absolutely loved this lecture. Kinda brings into context how vastly complicated the whole biological system is. This is so beautiful.

[TheAlexagius]

A great resource, especially during this COVID-19 pandemic

[beckysam]

Thank you for the presentation, it is a very good introduction to CRISPR and easy to follow!

[liptikantabhoi]

Dear Dr. David, I'm really excited for your work. Sir, I'm working on synthetic biology approach to increase sesquiterpene on bacterial system. Please suggest me some important things so that i can achieve my desired product in a large scale scale🙏🙏

[sabharabaya]

Fascinating. But in the experiment on antimicrobial resistant bacteria, if both resistant and sensitive bacteria grow on the same culture, how resistant cells acquire the vector for CRISPR while the other sensitive ones do not?🤔

[safdarsafdari]

why instead of using bacteriophage to weaken resistance bacteria to further using antibiotics they won't eliminating all of it by crispr?

[harshadannina]

Sir can you suggest a software tool to process DNA / RNA using a language like Verilog like CELLO. And also where can we find the data to conduct experiments?

[ayush]

Using CRISPR to kill bacteria. Ironic 😂😂