Phenylalanine Deaminase Test - Microbiology Class - Podcast (CC)

In this video, we explain the Phenylalanine Deaminase Test, which is used to determine whether bacteria can deaminate the amino acid phenylalanine using the enzyme phenylalanine deaminase. This test is important for identifying certain bacteria that produce phenylpyruvic acid as a result of this deamination process.

Key points covered:

Purpose: To detect the presence of phenylalanine deaminase in bacteria.
Procedure: Bacteria are inoculated onto a slant medium containing phenylalanine. After incubation, ferric chloride is added to detect phenylpyruvic acid.
Results Interpretation:
Positive: A green color indicates the presence of phenylpyruvic acid, confirming deamination.
Negative: No color change or yellow color, indicating no deamination of phenylalanine.
This test is crucial for distinguishing between different bacterial species. Watch to learn how to perform the test and how to interpret the results!