Breeding Cordyceps fungi | Multi-Ascospore isolation

Discover the fascinating world of Cordyceps militaris breeding in our step-by-step YouTube tutorial! Join us as we unveil the secrets to creating a new strain of this incredible mushroom.

In this comprehensive video, we guide you through the entire process, starting with isolating multi ascospore colonies on a water agar plate. Watch closely as we demonstrate the ingenious use of petroleum jelly to secure the mushroom to the top of the agar plate, allowing the ascospores to discharge onto the water agar below and germinate into monokaryotic mycelium.

Over a span of two weeks, witness the captivating union of mycelium as they mate together, forming dikaryotic mycelium. We then show you how to skillfully transfer the dikaryotic mycelium to a liquid culture, expanding its growth using a scalpel.

But the journey doesn't end there! In the final phase, we introduce the cultivated mycelium to a rice substrate. Witness the exciting results as we test the viability and potential of our new Cordyceps militaris strain.

Cordyceps Cultivation Tutorial and Related Recipes:

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#cordyceps #mushrooms #spores #medicinal #growyourown #mushroom #fungi #gardening #breeding #exoticplants #herbs #supplements #cultivation #hobby #microbiology #mycelium

[GreenTableGardens]

killing it man!!! Thanks for all you do for the community.

[iBuffy_]

Thank you for all you do. I'm a beginner and you've been incredibly helpful!!

[Allyourbase]

I really want to learn how to make different strains . This is really cool

[mulamoney]

This was amazing ❤ thank you 🙏🏾 for this, I really appreciate it man.

[MemoryPallace]

So to preserve it can you just dry them out? And what to you think the self life would be?

[sbpermaculturesilvopasture]

Hi, Nice work, and good continuation . I have a question, If I want to expand the LC to other LC how much time is too much, let say I want to inoculate a large scale bins how can I operate, thank's

[nevinedmundson]

How long can you store your LC in the fridge before degradation? I heard that it's best to use it within the first couple of weeks but was curious if you've attempted cultivation after longer storage

[SERGEYFT]

Do Cordyceps militaris spores last long? Can you use them to make a spore print on foil and store it for years?
Or should the spores be planted immediately since their shelf life is short?

[MisterMattis-roql]

tanks for de video, the seriinge can survive until Brazil? and how much each 10 ml of Cord Mili LC?

[jamiegaudet]

Are you able to do spore prints with cordyceps? Curious if there's a method of long term storage like spore printing for these

[klh]

Thank you for this video. If you have time to answer, what brand and size is that hepa filter?

[BAMBOOM]

Is that your etsy shop with the innoculation syringes?

[camsurbanfarm]

What would be the effect of using regular MEA instead of water agar for this?

[itsme-pt]

Hi and thanks for vid Q If my cord have overlay is the mycelium save to eat thanks

[Kathechan]

What is the temperature that you storage the plates?

[TheScientist]

This is what I was wondering if you were doing.

[momsmushroomsjodyfoster]

I would have thought 2 weeks of colonizing liquid culture to be way too long??