Method development and Identification of Triacylglycerols species with SFC

Analyzing Triacylglycerols (TAGs) is essential to evaluate the quality and functionality of oils and fats. Chromatographic separation by typical methods that include GC and HPLC might be challenging due to a large number of TAGs species. Supercritical Fluid Chromatography (SFC) is one chromatography method that uses supercritical fluid (i.e. supercritical carbon dioxide) as a mobile phase. Here, we introduce the workflow of method scouting of SFC using Method Scouting Solutions and how to establish the optimized analytical method for TAGs species in natural oils and fats.

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00:00 Start
01:26 The background of TAGs analysis
06:12 Nexera UC and Method Scouting Solutions
09:05 TAGs composition profiling in palm oil
13:48 Chiral separation of TAGs positional Isomers

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#Shimadzu, #supercriticalfluidchromatography, #SFC , #methodscouting