PCR Optimization and Troubleshooting

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  1. Rahul Patharkar
  2. pcr optimization
  3. pcr troubleshooting
  4. pcr problems
  5. pcr enzymes
  6. primer dimers
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Комментарии
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Greetings Rahul, happy to see you posting again! Your past videos have been a huge help to me. I am currently attempting to amplify a 6kb region using a 5x MasterMix and was wondering if you have any recommendations. I've tried several different combinations of annealing temperature and extension time but nothing seems to do the trick.

EDIT: After watching the last few seconds of your video, I realized I should probably try varying my enzyme 😅

jasondotgen
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10:28 - I disagree with the null hypothesis! There is no better DNA polymerase than Primestar GXL by Takara. My friend amplified the entire SARS-CoV-2 genome with it - 30 kb, no trouble shooting. GXL has never let me down and it's less expensive than Q5 (which is sooo finicky)

MattbyNature
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Do you have a direct PCR protocol for leaf tissue?

tapariayogesh
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can u tell me how to modify my normal PCR mastermix to conduct the multiplex PCR

EelasMusic
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