CD4 T lymphocytes presence revealed in the healthy mouse brain

Using confocal imaging on the mouse brain, Liston and colleagues identified rare CD4 T cells scattered across the brain, including cells beyond the glia limitans. 3D surface rendering of brain stained for CD4 T cells (green), CD31+ vasculature (red), GFAP+ astrocytes (magenta) and DAPI (blue). Representative images of CD4 T cells enclosed beyond the glia limitans in the cerebellum.

Pasciuto, E., Burton, O.T., Roca, C.P., Lagou, V., Rajan, W.D., Theys, T., Mancuso, R., Tito, R.Y., Kouser, L., Callaerts-Vegh, Z., de la Fuenteu, A.G., et al. (2020). Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition. Cell. 182.