Calculating Isoelectric Point of Proteins (Example)

[aarondesalvio]

Thank you so much, this was explained incredibly well! One piece of advice for anyone who's confused: keep in mind whether or not the side chain is neutral or positive in the protonated form. This is relevant for the COO- group, where it is NEUTRAL when protonated, as opposed to an NH3 group that is POSITIVE when protonated.

[JuanRomero-xelf]

YouTube Channels like this one-- (among a few others) are the future of learning. Thank you.

[lazer]

Very well explained, best video I have come across on youtube that explains this principle thus far

[thubelihlesithole]

I'm a bit confused, I'm not quite sure why you concluded the two acidic amino acids as neutral and not positive.

[charmainemoya]

Wow, you're a LIFE SAVER. While I spent hours on homework trying to read my book and notes and still scratching my head, you make everything clear in less than 15 minutes per lesson. Love all your videos!

[tylerlynn]

this guy is the only reason I made it to pharmacy school

[thepowerofpositivity]

Jus follow the simple rule if (1) the compound is a weak acid then the protonated form will be uncharged (AH) and the deprotonated form (A) will be negatively charged;(2) the compound is a weak base then the protonated form (BH) will be positively charged and the deprotonated form (B) will be uncharged. The tendency a compound will donate or accept proton depends on pH of the solution. Hope this help!

[reubent]

AK LECTURES Hey great video ..one thing that i'm a bit stuck on is why pka of 4.1 was neutral at pH 3.5 for the first example and why it was neutral for pka of 8.3 at ph7 in the 2nd example. thanks

[TonilaineC]

After watching this, I might just pass my biochem exam. Thank you so much 😭😊

[pjred]

Thank you soo much! My first Biochem exam is tomorrow and you saved me!!

[cambokid]

At first your videos were intimidating at first glance by the excessiveness, but omg it's very simple, straight to the point, and the explanation is beautiful, thank you!

[andrewcho]

Thank you so much! I got very confused on this section and my professor wasn't of much help on figuring out how to to this but looking at this, I realize that it is much more simple than we think!

[Horsinyears]

This was super helpful. I like that you explain the steps very clearly and make a point to separate them out.

[chillsteplife]

Amazing! Very easy to understand, very well organized! Cant say enough about how clear and concise your lecture is. Thanks for the video.

[britforlife]

I didn't understand how to calculate pI before this, but now I do! Thank you so much, it's much appreciated

[joseibarra]

Your videos are so helpful. Great for the new MCAT.

[catherinepaciotti]

Hi, thanks for the video. Did you forget to take into account the pka of cysteine?

[zay]

Thank you so much! I have my final exam tomorrow and this helped a lot! Hope u succeed in whatever you do.

[AP-ljqe]

amazing video, very clear explanation.  I am glad to watch this video because my professor explanation is sucked.

[penelopetaylor]

You are a true hero. THANK YOU for these amazing lectures.