Chapter 5.1: Protocols for PRF presented by Dr. Richard Miron

This video is a relatively short one presented by Dr. Richard Miron designed to simplify protocols for the production of PRF. In general, only three protocols are necessary for PRF therapy. The first is the standard PRF membrane in which ideally a higher platelet and leukocyte yield is harvested with an even distribution of cells within the upper 4- to 5-mL PRF layers. This is best achieved using a horizontal centrifugation system (700g for 8 minutes). The second protocol is a liquid-PRF formulation capable of concentrating platelets and leukocytes within the upper 1-mL layer (previously known as i-PRF). By utilizing a horizontal centrifugation system, higher concentrations are ensured (smaller volume though a higher concentration of cells). This protocol is achieved utilizing a 300g protocol for 5 minutes. The final and third protocol is that of Concentrated-PRF (C-PRF), where cells are purposefully accumulated specifically toward the buffy coat layer using faster spin protocols. This is best achieved using a 2000g protocol for 8 minutes; a resulting 0.3- to 0.5-mL cell-rich zone may be collected exactly within the buffy coat. With these three simple protocols, the practitioner is able to perform practically all clinical procedures as highlighted within this chapter.

Please leave comments below.

[angelaaston]

I appreciate this concise, scientific level instruction. As soon as I can afford this book I’m all in. Signed, A facial aesthetic need.

[austinjenkins]

Would the presence of anticoagulant effect the distribution and concentration of cells for a PRP protocol modeled from these studies?

[mayada]

Hi, what type of vacuum tubes need to be used for i-PRF liquid, also i would like to know at what speed and for how long with vertical centerfug??
Kind regards
Mayada

[gracech]

Hello, what are the recommended g forces for a fixed angle centrifuge? Thank you

[YenHai-ffcw]

I want to ask how long does the plasma gel keep and is there a way to melt it when injected into the body?

[galinasvagzdyte]

Hello. My centrifuge's rotor diameter is 95 mm. By formula given to spin 2000g converted into rpm is 4336 rpm. The max I can do rpm with my centrifuge is 4000rpm. In this case shall I spin 9 minutes instead of 8? Or enough 4000 rpm for 8min for CPrf? Many thanks

[alyaaismael]

Hi, excellent discussion, can i know the title of book? Can i get it as PDF. ?
Respect.

[ArnohChowdary-mblv]

What are the protocols for C-PRF with a fixed angle centrifuge?

[Dr-]

Its said that liquif PRF rrmain liquid for 8..9 mints then Why my PRF coagulated after 4...5 second after centrifuge and even 2 tubes of blood coagulated during centrifuge ??
Note ..i used tube with no lid cover
How to prevent this very earlier coagulation of PRF
thanx