Knockdown and Sphere Assay to Study Stermness Gene in GCSCs | Protocol Preview

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells - a 2 minute Preview of the Experimental Protocol

Jixian Xiong, Yuting Li, Xiangyu Tan, Li Fu
Shenzhen University School of Medicine, Guangdong Key Laboratory of Genome Stability and Human Disease Prevention, Department of Pharmacology and Shenzhen University International Cancer Center;

In this protocol, we present an experimental design using a conditional knockdown system and an adapted sphere formation assay to study the effect of clusterin on the stemness of patient-derived GCSCs. The protocol can be easily adapted to study both in vitro and in vivo function of stemness-associated genes in different types of CSCs.

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