Biuret Test for Proteins | Protein assay | Estimation of Protein | Dr. Nagendra Singh | PENS#38

#biuretassay #proteinestimation #labexperiment

The biuret test is a chemical test used for detecting the presence of peptide bonds in proteins an dpolypeptides. copper ions form coordination complex with peptide groups i proteins which absorb at 540nm. The biuret reaction can be used to assess the concentration of proteins because peptide bonds occur with the same frequency per amino acid in the peptides. The principle of the biuret assay is similar to that of the Lowry method, however it involves a single incubation of 20 min and takes lesser time than lowry assay.
The biuret is a good general protein assay for batches of material for which yield is not a problem. The Bradford assay is faster and more sensitive.
The main methods of protein estimation are:
1. UV based detection
2. Biuret method
3. Lowry method
4. BCA method
5. Bradford method

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[kitchenwithsara]

Spectrophotometre ka b btaye standard curve protein k liye

[arvindprajapati]

In Biuret Reagent, if we use KOH at place of NaOH then what will be effect of KOH ?

[piyushparmar]

The last calcution will be 17.6 because 0.5 ml of portein contain 8.8 so for 1 ml it would 8.8+8.8 so tha answer for last 1 ml of protein is 17.6. Can anyone explain me

[amritasingh]

Why Biuret method is less sensitive than Lowry method.

[mansichaturvedi]

Will the resultant colour vary between polypeptide (upto 50 aa) & protein sequence ( more than 50 aa)??

[mulhimmukhtar]

How BSA concentration(mg/ml) of test tube 1-5 is calculated?

[vidurchoudhary]

What would happen if we use ethylene glycol instead sodium potassium tartrate (Rochelle salt) in biuret assay?

[vidurchoudhary]

What will we observe if we reduce the alkalinity of the solution in biuret test?

[ibtkajalyadav]

Wht would happen if metal ion chelaters like EDTA are present in sample?

[JanhaviRai-IBT-]

How does the biuret reagents work to detect the protein?

[vishamberdayal]

If no biuret used in biuret reagents in protein estimation .so why it is called as biuret test for protein estimation?

[yubrajpani]

How.to calculate bsa concentration in mg/ml

[mukshy_]

Which free amino acid gives a positive biuret test?

[Lavanya]

What is the black precipitate formed when preparing biuret reagent?

[niharika]

why would amino acid give a negative result in the biuret test?

[arvindprajapati]

Why biuret method is very sensitive and rapid for protein estimation as compared to others methods?

[foodtechnologist]

Kindly reply me sir..is sodium potassium tartrate and potassium sodium tartrate same??
Because I have potassium sodium tartrate.

[kirti]

Is it possible to conduct biuret test to determine proteins for a wastewater sample?

[ibtkajalyadav]

Does biuret reagent react with amino acids?

[racingcar]

When we do experiment of buriet test, how does color change?