Gradient PCR

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Gradient PCR is a technique that allows the empirical determination of an optimal annealing temperature using the least number of steps. This optimization can often be achieved in one experiment.
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Hello we have pcr thermocyler of analytic Jena biometra and in my pcr machine gradient pcr option is not showing anywhere I m very confused how can I set the gradient pcr?

MICROTECT
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I think 64.6 is the perfect annealing because 62.3 itself showing two bands mearged which is not in 64.6....!

cutieaadi
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Thank you. I still don't understand about duplex pcr. Duplex PCR has to be optimized for annealing temperature because it uses two types of primers at once. Can you explain about the duplex pcr process?

noraisyahzulkifli
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Thanks very much. Please could make your audio a bit clear the next time

brightasante