High Throughput Homogenization of Plants webinar

High throughput homogenization methods have helped to eliminate a major bottleneck in HTS laboratories. Homogenization of plant samples, however, can be difficult due to the diverse biochemistry of the group and resilient nature of many tissues. Successful processing of plant samples requires a practical knowledge of the tools available for homogenization and an understanding of the sample itself. This webinar will include a discussion of the tricks and tools used for high throughput processing of plants, focusing on plate homogenizers. Sample type, such as leaf punches and seeds, greatly influences processing format and media type selection and will be covered at length. Cryogenic grinding, a technique often used for RNA isolation, will also be discussed.

Slides for the presentation:

00:00 Introduction
00:32 Announcements
02:49 Our Interests
05:22 Pycnidia Sporulation
06:11 Our Interests (cont.)
06:56 Defining High Throughput
08:54 Microplates - Highly Defined
10:03 Options for Homogenization
12:21 Bead Beating
14:53 Mixer Mills are for Bead Beating
17:08 High Throughput Homogenizers
18:25 Popular HT Homogenizers
20:35 Geno/Grinder Series
21:42 Tissuelyser & HT Homogenizer
22:33 Stroke Considerations
26:13 Vessels
32:21 Plate Considerations
35:59 SBS Format Vials
37:08 Sample Types
39:08 Target
42:50 Difficulty with Seeds
45:09 Biochemical Diversity
47:10 Processing Options
48:25 Dry Grinding
49:57 Wet Grinding
52:39 Effect of Polyphenol Oxidase
53:45 All Grinding Shears DNA
56:37 Cryogenic Grinding
57:45 Cold Sinks Help
58:42 Summary
1:00:40 Q&A
1:05:06 Conclusion