Time-lapse of cell migration and apoptosis during angiogenesis

Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. CellTracker™ Red CMTPX, a red-fluorescent cell tracer, was used to label cultured human umbilical vein endothelial cells and follow their migration in an in vitro model of angiogenesis over a period of 40 hours. The cells were also incubated with CellEvent® Caspase-3/7 Green ReadyProbes® Reagent, an indicator that is internalized by cells and fluoresces green when cleaved by activated caspase 3 or caspase 7, early markers of apoptosis. The time-lapse images were captured and assembled using an EVOS® FL Auto Imaging System equipped with the EVOS® Onstage Incubator.