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Endotoxin (ET) ELISA Kit (SKU: UNEB0040) - ELISA Genie
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Endotoxin (ET) ELISA kit can assay for Endotoxin (ET) in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. This video describes an Endotoxin (ET) ELISA kit. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Endotoxin (ET) is proportional to the signal generated by the reaction meaning the Endotoxin (ET) ELISA Kit assay gives you a quantitative measurement of the analyte in your samples.
Find out more here:
Get in touch:
Phone: + (44) 2081237624
More ELISA Genie Videos:
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. This video describes an Endotoxin (ET) ELISA kit. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Endotoxin (ET) is proportional to the signal generated by the reaction meaning the Endotoxin (ET) ELISA Kit assay gives you a quantitative measurement of the analyte in your samples.
Find out more here:
Get in touch:
Phone: + (44) 2081237624
More ELISA Genie Videos:
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