Analytical Instrumentation 06: Fluorescence & Phosphorescence Explained | Learn under 5 min

Welcome to Episode 6 of our "Analytical Instrumentation" series! 🔬✨

In this concise 5-minute animated video, we delve into the basics and principles of Fluorescence and Phosphorescence measurement—essential techniques in qualitative and quantitative analysis within research laboratories, QC labs, and the field of analytical chemistry. Whether you're a student, researcher, or laboratory technician, understanding fluorescence and phosphorescence is crucial for accurate and efficient analytical work.

📚 What You'll Learn in This Video:

Introduction to Fluorescence & Phosphorescence:
Definitions: What do fluorescence and phosphorescence mean?
Common Questions Answered:
What does fluorescent mean?
How does fluorescence work?
How does fluorescence spectroscopy work?
How does phosphorescence occur?

Energy Level Diagrams:
Molecular Energy Levels: Understanding different levels of molecular energies.
Singlet & Triplet States:
Singlet State: Electron pairing and spin configurations.
Triplet State: Differences from singlet states and their significance.
Electron Pairing & Spins: How electrons pair and their spin orientations in different states.

Mechanism of Fluorescence & Phosphorescence:
Fluorescent Emissions: How electrons return to the ground state, emitting light.
Phosphorescent Emissions: Delayed emissions due to triplet state transitions.
Energy Transfer Processes: How energy levels facilitate light emission.

Applications in Analytical Instruments:
Spectrofluorometer: Introduction to the instrument used for fluorescence and phosphorescence measurements.
Qualitative & Quantitative Analysis: Using emitted light to determine analyte concentrations.
Comparative Analysis:

Differences Between Fluorescence & Phosphorescence:
Emission Lifetimes: Immediate vs. delayed emissions.
Energy Transitions: Singlet vs. triplet state transitions.
Applications & Advantages: When to use each technique for optimal results.

🔍 Key Topics Covered:
Fluorescence and phosphorescence fundamentals
Energy level diagrams and molecular states
Mechanisms of light emission
Spectrofluorometer operation
Beer-Lambert's Law in quantitative analysis
Comparative analysis of fluorescence vs. phosphorescence

🎯 Target Audience:
Students and professionals in analytical chemistry
Researchers and laboratory technicians
Individuals interested in the mechanics of analytical instrumentation
Anyone looking to deepen their understanding of scientific instrumentation and analytical techniques
Stay calm and keep learning! Whether you're a student, researcher, or enthusiast, this tutorial will equip you with the knowledge to master fluorescence and phosphorescence measurements effectively—all through engaging 2D animations!

🔔 Don't forget to like, share, and subscribe for more insightful and educational videos on analytical instruments and engineering concepts!

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on Spectrofluorimeter

on Double Beam Spectrometer

on Basics of UV-Vis spectroscopy in Analytical Instrumentation

on Filters and Monochromators, Gratings used in Analytical Instrumentation

on Detectors used in Analytical Instrumentation:

on Single Beam Photometer used in Analytical Instrumentation
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Link to the video on Accelerometer:

Link to the video on Gyroscope:

Link to the video on Magnetometer:

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Please watch our playlist on Digital Electronics:

Please watch our playlist on Analytical Instrumentation:

Please watch our playlist on Automation and Programmable Logic Controllers (PLC):
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Credits :

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[bosong]

I really like the video but I would like to suggest that you remove the background music. It is distracting!

[vinayegalapati]

You're explanation and PPT was awesome 😍

But, Please decrease background volume

I'm unable to concentrate on the concept

[soumyamuttagi]

How I couldn't find this video earlier. Really amazing and beautiful explaination. Finally I can score and understand at the same time 🙌🙌🙌thank youuu

[Alex-vsev]

Man, I'm from Germany. And idk what the hell is going on with our teachers but I understood this topic better in not even 5 minutes than in 2 weeks of school lessons. And your video is in English !!!

[cherryberry]

Good explanation.But music is make concantrate difficult.

[pritykarmakar]

Very helpful video, thank you so much sir 🙏🙏🙏😊😊😊

[TestEric]

WOW, amazing job, this is exactly what I was looking for. I'm shocked more people are not interested in this topic.

[LadyShaydex]

I think you should add why fluorescence light is higher in wavelength, due to energy being lost to vibration

[الجبلالموصلي]

The 1st second I was like "music😫", but then I was into the mood, thank you so much, I was having real trouble with singlet and triplet state 🌺

[chitrakeshwani]

thank you so much for making this video you have explained this phenomenon in a very simple and comprehensive way

[safwanahrahman]

simple explaination and i understand the whole thing better!!! THANKYOU

[savitact]

thankyou. very well explained. it would me better if no background music.

[minhavidavai]

This was great. I needed to remember these concepts in short time. Thanks

[HamnaAzhar]

Beautifully explained... Thank u for making it quite easy to understand!

[ncarti]

Great video, really liked the visuals and it helped me understand it more!

[sowjanyaponnada]

Nice and telugu explanation... Is better to understand

[amitvele]

Excellent work. Colorful and creative. Makes the video engaging.

[harshitabhati]

Awesome!! I can never forget the concept after watching this!!

[vrajpatel]

Very nice discription very good way to explained keep it up

[innocentchemist]

Good work. it was really helpful for me. this video cleared my basic concepts!!