How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)

This step-by-step technical guide demonstrates how to isolate peripheral blood mononuclear cells (PBMCs) from whole blood using density gradient centrifugation (e.g. Ficoll-Paque™ or Lymphoprep™).

In this video, you can see how to layer blood over the density medium and learn two different ways of harvesting cells from the blood plasma: density medium interface.

#PBMCisolation #CellSeparation #CellIsolation

[crostail]

Hi! Is there any reason why the whole blood preparation and the Ficoll shouldn't be mixed before centrifugation? I don't quite undestand this phase because in the centrifugation, they will be mixed anyway. Thanks!

[samaa___]

I dont access to a centrifuge with the brake off, can i isolate pbmc with this condition?

[mathildejbb]

Thank you very much for this very nice video. I am currently following the same process but with sheep blood. The point is I don't have 4 layers after the first centrifuge. Does anybody knows if the type of blood plays an important part in this experience ? thank you to reply me

[WeerinThammachote]

If I just use whole blood that hasn't been diluted with PBS, will the protocol be the same?

[marializziecuellar]

Interesante video. Muy didactico y reproducible para el pregrado.

[MozhdehHaj]

Can we use ficoll insted of histopaque solution?

[elledee]

I Purchased PBS from stemcell tech, what do i need to add to make it 2% FBS.
What will be the problem if we use only PBS w/ no 2%fbs?

[pradeepleo]

How much ficoll is to add for 5 ml blood with 5 ml PBS.

[بثينةبثينة-لس]

How is a lymphocyte isolated from whole blood?