Retrieving Sequence - Complement and Reverse Complement - Bioinformatics Practical 1

#Retrieving_sequence_in_correct_orientation - In this lecture, I describe the basic concept of Sequence - #Plus #strand, #minus #strand, #complement and #reverse #complement and also explain the steps to #retrieve the sequence of the gene from #UCSC #genome #Browser

#sequence #plus_strand #minus_strand #complement #reverse_complement #UCSC_Genome_Browser #lets_grow_together #vipins_e_classroom #bioinformatics #biotech

[Sing-with-Amna]

Respected sir
I am a biotechnology student and being a biotechnologist i personally feel that a biotechnologist must be expert in bioinformatics tools.
I have knowledge of wet lab but not of dry lab
I have taking the lectures from your lectures series and you have taught in very good way.
Thanks you and keep it up

[AmitaBarik-dr]

Very nicely explained Sir. If we do not mask the repeats then also it will be shown in the DNA sequence ?

[ranakamei]

Thank you, Sir, for your clear explanation of the reverse complementation. But I am confused. How do we determine a DNA strand as either + or - (plus or negative) strand? What is the reference to it?