Intro to Light Microscopy 2: Principles of Fluorescence Microscopy

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In this module you will learn about fluorescence and how it is used to generate high contrast microscopy imaging.

Learning Objectives Include:
• Properties of light
• Absorption and emission and how these relate to contrast and fluorescence techniques
• The mechanism of fluorescence
• A Jablonski diagram and how it shows the mechanism of absorption and emission of light by molecules
• Stokes shift
• Mechanism of quenching
• How filters work

Presented by Eleanor Kable from Sydney Microscopy and Microanalysis, a Microscopy Australia facility.

These videos provide guidance only. Local work health and safety protocols should always take precedence when you undertake any lab procedures in your own facility. Some of the videos show workflows for specific instruments and their content should not be taken as specific instructions for other types of instruments.
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