TA cloning method in HINDI EASY EXPLANATION IN 5MINUTES!!!!! #tacloning #tacloningsteps #topocloning

TA cloning is a subcloning technique that avoids the use of restriction enzymes and is easier and quicker than traditional subcloning. The technique relies on the ability of adenine and thymine on different DNA fragments to hybridize and, in the presence of ligase, become ligated together.
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[sheelababu]

One doubt, which one is correct either 5 ' T overhang or 3'T overhang. In figure u said that 5't overhang but in sentence it is 3' overhang.