Setting Up An RNA-seq Experiment | Pluto Bio

Bulk RNA sequencing (RNA-seq) is a workhorse of biology, enabling us to investigate genome-wide gene expression levels (i.e. transcriptomics). This in-depth tutorial walks you through the step-by-step process of setting up a new RNA-seq experiment in Pluto, uploading data, and kicking off a pipeline to convert FASTQ files into a count matrix.

After the pipeline completes, come back to check out the second video in the tutorial for instructions on how to view the QC report and perform downstream analysis.