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Primary Myoblasts Isolation and Differentiation | Protocol Preview
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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants - a 2 minute Preview of the Experimental Protocol
Megan Vaughan, Katja A. Lamia
Department of Molecular Medicine, Scripps Research;
Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.
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