Altogen Labs HCT116 Xenograft Service Colon Cancer

The HCT116 colon cell line is utilized to create subcutaneous and metastatic xenograft colon cancer mouse model HCT116, a widely used CDX (Cell Line Derived Xenograft) in colon cancer research for the evaluation of tumor growth inhibition of a single therapeutic agent such as docetaxel, 5-FU, flavopiridol or in combination with targeted agents.
Xenografting is the transplantation of tissue from one species into another.
Xenografting has been established as benchmark studies in pre-clinical cancer research.
Typically, immunodeficient mice serve as hosts for a wide variety of human tumors, effectively serving as models for human subjects.
Xenografting is a complete and accurate study of tumor growth and the activity of drug administration.

-HCT116 Xenograft Model
Sample Cell Lines Available

-HCT116 Xenograft Model
Advantages of Xenografting
Develop new therapeutic agents quickly, efficiently and cost-effectively
Evaluate the efficacy and toxicity of potential therapeutic agents
Evaluate target compound activity using in vivo system (human cells)
Predict cytotoxicity of cancer drugs

-HCT116 Xenograft Model
Basic Xenograft Study
Xenograft studies can be designed to:
Identify lead compounds
Optimize dose schedules
Identify combination strategies

-HCT116 Xenograft Model
Routes of drug administration:
Intratumoral
Intramuscular
Oral gavage
Intravenous
Intratracheal
Subcutaneous
Intraperitoneal
Continuous infusion
Intranasal
Using cutting-edge micro-injection techniques

-HCT116 Xenograft Model
Following options are available for the HCT116 xenograft model:
HCT116 Tumor Growth Delay (TGD; latency)
HCT116 Tumor Growth Inhibition (TGI)
Dosing frequency and duration of dose administration
HCT116 tumor immunohistochemistry
Alternative cell engraftment sites (orthotopic transplantation, tail vein injection and left ventricular injection for metastasis studies, injection into the mammary fat pad, intraperitoneal injection)
Blood chemistry analysis
Toxicity and survival (optional: performing a broad health observation program)
Gross necropsies and histopathology
Positive control group employing cyclophosphamide, at a dosage of 50 mg/kg administered by intramuscular injection to the control group daily for the study duration
Lipid distribution and metabolic assays
Imaging studies: Fluorescence-based whole body imaging, MRI

-HCT116 Xenograft Model
Altogen Labs’ team of scientists carries extensive experience in Xenograft model research.
Altogen Labs is compliant as a Good Laboratory Practices (GLP) environment, which is required by the FDA for pre-clinical research.
Our Altogen Labs’ Xenograft services are extensive, rigorous, and flexible.
Contact us to discuss Xenograft model details, timeline estimates, and price quotes!

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