SCOG Virtual Lecture Series - Gabriela Salinas (University Medical Center, Göttingen)

"Technical Improvements in Single-Cell RNA-Sequencing delivering the promise of RNA therapeutics"

Single-cell multi-omics analysis has the potential to yield a comprehensive understanding of the cellular events that underlie the basis of human diseases. The cardinal feature to access this information is the technology used for single-cell isolation, barcoding, and sequencing. Most currently used single-cell RNA-sequencing platforms have limitations in several areas including cell selection, documentation and library chemistry. In this study, we describe a novel high-throughput, full-length,
single-cell RNA-sequencing approach that combines the CellenONE isolation and sorting system with the ICELL8 processing instrument. This method offers substantial improvements in single cell selection, documentation and capturing rate. Moreover, it allows the use of flexible chemistry for library preparations and the analysis of living or fixed cells, whole cells independent of sizing and morphology, as well as of nuclei. We applied this method to dermal fibroblasts derived from six patients with different segmental progeria syndromes and defined phenotype associated pathway signatures with variant associated expression modifiers. These results validate the applicability of our method to highlight genotype-expression relationships for molecular phenotyping of individual cells derived from human patients.

Biography:
Gabriela Salinas is heading the NGS- Integrative Genomics Core Unit at the University Medical center Göttingen since 2007.
After her studies in Biochemistry at the Universidad de Ciencias Quimicas in Cordoba, Argentina she started and completed her Ph.D. in Human Genetics at the University Hamburg-Eppendorf (UKE).
After her Ph.D she head a Laboratory PK/ADME using artificial Intelligence based Drug Discovery at the „NSCI Novel Science International GmbH”, Göttingen and in 2003 started leading projects for Pharma companies in the field of toxicogenomics at the Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, Hannover.
Research interests include the NGS-based technologies for genomics, transcriptomics, and epigenomics that are now increasingly focused on the characterization of individual cells in the field of genomic medicine e.g. the optimization of methods for single cell approaches, improving data quality and muti-omics data analysis.

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