29. Cell Imaging Techniques

MIT 7.016 Introductory Biology, Fall 2018
Instructor: Adam Martin

Professor Martin introduces cell imaging techniques, which are tools that allow biologists to observe what's going on living cells. He goes over several types of microscopy and discusses how each deals with the challenges of resolution and contrast.

License: Creative Commons BY-NC-SA

[CodeJeffo]

Regarding the resolution formula. I believe one has to consider rather the wavelength of the emission light not the excitation light. If you take the excitation wavelength that formula tells you to what smaller possible spot you can focus that light to. Vice versa if you have 2 GFP fluorophores emitting at certain wavelength this is what's important not the excitation light. Otherwise we would not observe worse resolution the further red we go with fluorophores.

[jakeeutis]

What a neat technique, no wonder he got a Nobel prize

[carolynlambert]

took intro to biology at tidewater community college from here go anywhere!

[luvisacigarette]

Is there an explanation of multiphoton microscopy?

[MaeLSTRoM]

I think it's funny that the view count decays rapidly from lecture 1 through to 29, and then suddenly there's a spike of interest on lecture 30 followed by another decay.
I wonder if it had anything to do with the current state of the world..