Actin filament assembly

Related links:

[toxikc]

People. Profilin inhibits nucleation and accelerates polymerization. These two are distinct

[ajhamton]

no audio?...i swear i checked my speakers..

[rickykay]

amazing video, explanation is so clear! I finally got it :)

[Mechanobiology]

Hi, thanks for your comment.

Actin filaments are polarized. Monomers bind to the filament at the '-ve' end and hence filaments grow in one direction - that is from their 'barbed end'. This first requires the 'capping proteins' to be displaced from the end of the filament. If elongation factors like formin are involved, they also 'add' monomers to the same end.

You can read more on actin polarity, capping proteins & the critical concentration of G-actin in the links listed in the description

[tarakamaliasl]

Profilin proteins promote polymerization and growth, not inhibition. This is wrong.

[LikeaBosstoday]

quick question, when F-actin is in the steady-state and is treadmilling, are actin monomers still getting added to the -ve end? I know that there is net growth at the +ve end and net loss at the -ve end, but are there still some subunits being added at the -ve end?

[MrHumberto]

preferential addition of actin monomers to (+) end, not (-) end

[MsJoanaPaula]

Very nice video :) Made me understand the subject!

[Eastvillage]

Nice video, but it didn't show much about critical concentration... and can polymerisation only go in one direction and not the other way?

[kevins]

What? This looks trippy but has nothing to do with real protein physics.

I think that that might just be someone's animation 'reel.'

[emad]

WRONG profilin promotes actin polymerisation and cofilin inhibits actin polymerisation