Supercritical Chromatography Chat: Faster, Greener Achiral Separations

In our Supercritical Chat Series, BUCHI experts cover topics that help you better understand how supercritical fluid chromatography (SFC) can work for you.   

During this episode of Supercritical Chats, our experts discuss the benefits of using SFC for achiral applications and provide a real-life example comparing the performance of flash plus prep HPLC against SFC using a ubiquitous sweetener, Stevia.  (Spoiler alert: SFC took 2 hours less and used about 1/8 of the solvent of the LC method.)    

If larger sample loading, higher throughput, and lower solvent costs are on your purification lab wish list, SFC could be a good fit for your lab’s achiral separations and more.

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00:00 Introduction
01:07 Quick review of SFC
02:15 What are achiral separations?
03:26 Why switch from LC to SFC for achiral separations
04:34 How to screen conditions for a novel separation using SFC
05:45 Choosing a column for SFC separations
07:10 Mobile phase modifiers for SFC
08:27 Stacked injections to increase SFC throughput
09:20 Stevia extraction case study
11:03 Stevia extraction case study
12:13 Gentle conditions for sensitive compounds
12:43 Detection options for SFC
14:05 Strategies for injecting hydrophilic compounds
15:19 Injection solvents for hard to dissolve compounds
16:01 Metal catalyzed chemistry tolerance