Selective PCR Amplification of AFLP Marker #geneticteacher

#Selective_PCR_Amplification_of_AFLP_Marker
Component of selective PCR amplification: Diluted DNA template from pre-selective PCR reaction and ECOR-1 with selective nucleotide (AAC) and Mse-1 with selective nucleotide (AAC) and dNTPs and 10X PCR buffer and MgCl2 and Taq DNA polymerase and deionized water, Selective PCR reaction include two phases (First phase involve 12 Cycles with decrease temperature by 0.7 degree Celsius) while (Second phase include 23 Cycles) and after finish all phases add 8 microliters Formamide loading buffer to PCR product
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