Agar well diffusion assay

Sensitivity test by well diffusion method.

[kabitaneupane]

The sample extract as well diffusion, and the positive control or standard as disc diffusion in the same plate. Can we differ like this sir?? Just curious to know 🤔

[goodnessonukwube]

Sir, I want to know, when one is using a plant extract, unlike conventional antibiotics that have a standard, how can one determine the concentration of the plant extract that inhibits the bacteria when using MIC

[ayasya.]

should the plate place inversed or not when incubated?

[TheYuenKim]

Hi Mr. Rejo. This is such a good and educational video. I just want to ask, what were the amount of melted agar and extract that you used?

[alexmares]

That you very much, very well explained.

[jessadesoloc]

Thank you for the video! May ask in what book can I find this step-by-step process? Just so I can put a reference book for an activity for school. Thank you!

[babusheikh]

Dear Sir, would you please tell me about how to prepare the sample? In my case, when I filtered my sample through 0.2 micron filter inside a laminar flow cabinet, my sample's concentration as well as the volume also get decreased. So, I am now in confusion about how to prepare the sample correctly. Please help!!

[Liki_Bu]

thanks for good video. does big size of well have the more powerful antimicrobial activity? or small size of well ?

[banupriyabanupriya]

Sir in comments, you said that if the extract is viscous, we dont need to add melted MH agar at bottom, in your experiment also the extract is viscous then why you added MH agar on bottom?

[KristelleMaeTardecilla-ehci]

Hi what is the diameter of the well using blue pipette tip

[anjua]

Sir, in the case of using sterile disc is it possible to add 100 microlitre on a single disc???

[lilyrose]

Hello Sir, what media you sugest for antibacterial testing for staphylococcus aureus?

[vikasashraf]

Dear sir
How to measure the ZOI in agar well diffusion method. since incase of Disc Diffusion assay the ZOI is measured end to end which also includes the area under the disc. in this case there is a well and couldnt be counted as clearing zone

[anjhere]

Thank you for the video sir. What control disc have you used in this video sir? I have a similar experiment with curcumin nanoparticles, but they are in paste form, not liquid form, do you have any suggestion in how I should load my sample inside the wells?

[salettedepintosouza]

Hello my products are neem extract nutmeg extract combo of neem & nutmeg extract herbal gel & marketed preparation. My need extract is viscous with what solvent I should dilute with?? Tween 80?? Can't I add above products

[chemist]

How to perform antibacterial activity of CuO nanoparticles

[anjanaramesh]

Sir, Which is media is used for sauropus androgynus plant extract for antibacterial test?

[halamohammed]

What is the composition of Muller Hinton Agar media?

[tribikramdahal]

Sir can you please make video on aloe gel extraction

[happychemist]

what is the name of possiitive control? and what is it purpose?