Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling- Adsorbed Protein Layers l Protocol Preview

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces - a 2 minute Preview of the Experimental Protocol

Laura A. McKiel, Kimberly A. Woodhouse, Lindsay E. Fitzpatrick
Queen's University, Department of Chemical Engineering;

This protocol provides researchers with a rapid, indirect method of measuring TLR-dependent NF-кB/AP-1 transcription factor activity in a murine macrophage cell line in response to a variety of polymeric surfaces and adsorbed protein layers that model the biomaterial implant microenvironment.

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