Culture Media (Part 1)- Animal Cell Culture

What is culture media ? It is a nutrient preparation that contains essential nutrients required by cells for their growth.

Two types of culture media :
- Natural media
- Artificial media
Natural media consists of naturally occurring biological fluids such as plasma clots, biological fluids like amniotic fluid, insect hemolymph, serum, tissue extracts.

Artificial media are grouped as:

- Basal media
- Serum media
- Serum free media
- Protein free media

Basal media - also termed as chemically defined media as all the components are known.

Components of Basal Media includes:
- balanced salt solution : provides an environment that maintains the structural and physiological integrity, pH and osmolarity of cells in vitro
- buffering system : regulates pH. these are of two types, natural and chemical
- energy source : commonly used is glucose. other sugars like maltose, sucrose and fructose are also used
- amino acids : increase cell proliferation.
- proteins/peptides : albumin act as binding protein, aprotonin act as protective agent and fibronectin aids in attachment of cells to the substrate
- growth factors : act as signal and helps in cell proliferation and differentiation
- vitamins : act as coenzymes involved in cell metabolism. eg vitamin B complex
- trace elements : copper, zinc and selenium are elements needed in micronutrients. These are essential for biological processes
- antibiotics : prevents contamination
- phenol red : serves as a pH indicator

WATCH the next video for Information on SERUM MEDIA, SERUM FREE MEDIA and PROTEIN FREE MEDIA

[rukhsarbukhari]

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[akhildhiman]

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[.akankshasonkarxlla]

Ap ki viedo best u tube pr ap se acha koi bhi bio tech nhi padha sakta h mam

[.akankshasonkarxlla]

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[vaishalichouhan]

Mam mujhe practical Mila hai.. prepration of animal cell culture media..to mam mai kaise use bnau?

[brennanjones]

With your knowledge, is it possible to create a media from certain plant cells for the process of cultured meat? Reason I ask is that a plant cell culture company mentioned supplying meat cell culture companies with plant material, and I can’t figure out how and why?

[.akankshasonkarxlla]

Mam plz recombinant DNA technology pr video laye i need help u mam