Hydrophobic interaction chromatography ( HIC) | Application of HIC

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Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. HIC is a useful separation technique for purifying proteins while maintaining biological activity due to the use of conditions and matrices that operate under less denaturing conditions.
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The visuals are great! They were so helpful for my understanding. Thank you.

qetsiyah
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I used to have a problem understanding HIC but watching this video has made me realize that I also have a problem understanding English.

sasukeuhiha
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Do you know how long should my HIC column be to isolate GFP from 1g e.Coli?

qamaral-ubiedi
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All the videos on this channel are very very useful. Thank you.

shivaniathani
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Great explanation, thank you very much!

vg
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So basically the hydrophobic analytes take longer to be detected as they interact more with the hydrophobic column?

JJH
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Thank u so much sir
I had problem in understanding hw they will use HIC for virus purification...now it's

suvismaya
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please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??

sharwtteer
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Sir if i purchase your course then will i be able to get paid videos of other teachers of iit jam

himankjain
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Clear and fluid explanation, thank you . greets

valentinascatizzi
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Need to increase volume a bit .
Thanks for the vdo

aparnaaa
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How can water molecules mask the hydrophobic surfaces???

rukhsarfatima
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Little bit clarity is needed while speaking rest the video was really nice and explanation through diagrams is good.... Keep it up 👍

shrutikachaudhary
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Nice but plzz make videos on animal and plant biotechnology important topics

richajoshi
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Explanation could have been more better with written texts.

rashmiranjansahoo
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i think u have problem in your voice... kindly focus on it.
and content is good but only voice.

hammadak
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Bro love this video but its more than 5 mins lol

hongchen
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please does better in which don't mine time limitation..

nanhelalgupta
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Such a low pitch sound really irritating and such things makes the learning distracted. Audio quality always matters when we try to maintain concentration.

kuldeepbiotech
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