Mammalian expression vector

This recombinant DNA technology lecture is to explain about the properties of mammalian expression vector and the details of the construction of mammalian expression vector. This lecture also explains the importance of using the mammalian expression vector.
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[ruhidyusifov]

Universites and professors need to follow Shomu`s way of explanation he is really brilliant. Thank you teacher!

[samyuktha]

I really need to thank u, ur way of explanation is great. Ur vedios really helped me

[Natalia-wdye]

Thank you for this video, it's hard to find info on this specific topic in free access and also you explain it well so it's easy to understand :D

[sanjeevakumar]

I request you to make a video on baculovirus vectors. By the way your tutorials are simply amazing

[ren]

Just a question. So this expression vector will not need to be integrated into a chromosome via homologous recombination for it to be expressed? It can just be introduced into the nucleus of the mammalian cell?

[Garcia-elf]

Can you explain what Markov chain Monte Carlo is and how it is applied to genomics??
What does "sample the joint posterior distribution" mean? Thanks

[sidrah]

Plz do a video on bucculovirus expression vector system in a bsc level

[janakirawool]

Is there any video on promoter selection with plasmid pKO1? ..I can't understand that promoter selection in pKO1

[livelovelaugh]

Question- when would u use prokaryotic expression vector and when would u use eukaryotic expression vector? Like in what situations is one better than the other?

[rhythmdancebymanisha]

What are the different kinds of vectors used in stable and transient transfection in mammalian cells and plant cells????

[SWyrick]

which restriction enzymes are required to clone the mammalian expression vector pMV2 BMPR?

[Kaoriocha]

How do transiently transfected plasmid in the cytosol get expressed? I thought the transcriptional machineries is inside the nucleus...

[manaspaul]

Sir please make a vedio about T7 promoter based vector

[KingLewis]

Poly-A tag sequence is not for protein isolaton!( 8:16 ) poly A tail is not expressed into protein so you can´t used to fish your protein.

[subhiksharajan]

Please do videos on bpv based vector and insect vector

[berastainisabel]

Great video, thank you very much for your clarity!! yet, poly A is not a selection tag, be careful!!!

[itratzahra]

very nice lectures. kindly suggest any book of gene cloning as easy and brief like ur lectures :)

[anicatasnimprotity]

can a mammalian expression vector be 'transformed' into a bacterial cell?

[mayukhroy]

Thank You Sir, for such a clear explanation.

[santoshitekam]

Sir please explain N-terminal
p-FLAG-Mac p-FLAG -ATS