Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography

This case study demonstrates an efficient purification workflow for a low-expressing recombinant protein. Multiple chromatography resins were screened for the capture step of this recombinant protein with goals to minimize fermentation dilution and increase purity and recovery to greater than 75%. Optimal wash and elution conditions were determined using a design of experiment (DOE) approach. An elution buffer containing NaCl and magnesium chloride was found to purify the target molecule with high recovery and purity.

Overview
- Importance of screening multiple resins
- DOE for optimizing purification and elution conditions
- Evaluation of modifiers for enhancing purity and recovery

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