Subtractive Proteomics for Identification of Drug Targets in Bacterial Pathogens: A Review

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IJERTV9IS010169
Subtractive Proteomics for Identification of Drug Targets in Bacterial Pathogens: A Review

Shalini Maurya , Salman Akhtar , Mohammed Haris Siddiqui , Mohammed Kalim Ahmad Khan

The first step in drug and vaccine discovery is the identification of the target. Subtractive proteomics can be widely used for the process. This approach has been used to find drug targets for the pathogen, which are resistant to drugs and for which there is no vaccine. This insilico method can reduce the cost and time for finding the drug targets. It involves subtraction of proteins of host and pathogen which provide a set of proteins, which are essential for pathogen but absent in gut flora and host. A review was carried out on a subtractive proteomics approach on twenty-nine pathogens, as reported from 2008 to 2018. These organisms have been categorized under two categories: Multiple drug-resistant organisms and those which have no effective drug to date. By subtractive proteomics the total proteome undergoes a subtraction process at each step, to narrow down to few drug targets. Tools and databases for subtractive proteomics have also been described.