Webinar (Preview): Utilizing Synthetic DNA Technologies in Antibody Discovery and Optimization

Utilizing its proprietary DNA technology to write synthetic libraries, Twist Biopharma enable the discovery of functional GPCR (G protein-coupled receptor) antibodies.

They grafted a large number of GPCR-binding motifs into a focused antibody library. By incorporating these motifs into the antibody heavy chain CDR3, they developed a first generation GPCR library.
They also mined patented GPCR antibody sequences, and used the sequence information to guide the design of another synthetic library. Twist Biopharma demonstrate the utility both libraries to discover potent functional antibodies against multiple GPCR targets.

0:15 and in particular I’m going to be highlighting how we use the iQue screener for high throughput cell binding analysis for all the antibiotics that come out of our libraries

0:28 to start off we do some gating strategies shown here just for this Adora 2A GPCR target where we overexpressed in a stable cell line and again we also GFP tag that that GPCR so we can basically gate on the GFP positive cells to which again should overexpress and express that particular this is that particular target which in this case is the Adora 2A

0:58 from our antibiotic optimization workflow which also uses the iQue screener I won't show any data from the tile platform as it relates to the iQue screener but I’ll just throw it in there because it's an important part of our overall process here at twist biopharma

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