Tutorial: How to Reverse Pipette?

Learn how to perform reverse pipetting, an alternative pipetting mode to the widely used forward pipetting technique. Reverse pipetting is particularly useful when working with viscous liquids such as glycerol or volatile liquids likr ethanol or isopropanol, and when pipetting small volumes.

Reverse pipetting involves pressing the pipette's operating button all the way down to the second stop before aspirating liquid into the pipette tip, allowing for an excessive amount of liquid to enter the tip. To accurately pipet the solution or sample, the operating button is pressed to the first stop when dispensing, and the excess amount can be returned to the original sample tube or discarded.

Electronic pipettes offer pipetting modes in addition to forward and reverse pipetting, most of which are based on the reverse pipetting technique where excess liquid is used to make multi-dispensing to a multiwell plate more accurate, or sequential dispensing for creating dilution series. When using reverse pipetting with aerosol barrier filter tips, it is important to ensure that the filtered tips have enough space for the excess volume, allowing for the full volume of the pipette and tip to be used.

Sartorius Pipetting Academy

Do not forget to subscribe to have better access to our videos!

#pipette #tutorial #howto

[pratisthitabaruah]

Thank you for introducing me to this new technique. I want to know that after dispensing a reagent into a reaction mixture by reverse pipetting, if we discard the excess reagent would it be a wastage of the reagent as we also can't return it to the original sample as it can contaminate the stock? How to overcome this problem?