Introduction to SMRT Sequencing

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A brief animated introduction to Pacific Biosciences' Single Molecule, Real-Time (SMRT) Sequencing, including the SMRT Cell and zero mode waveguide (ZMW).

Note: NO AUDIO in this animation. Explanatory text is included.

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Jonger Chua: the way I understand it is that the size of the chamber is such that the illuminating light pulse only passes far enough into the wave guide to excite the markers which then emit their frequency out the guide at the top to the detector. If the illuminating pulse were to continue through the waveguide the detector would essentially be "blinded". This is how they achieve the high signal to noise needed for accurate recording.

stserviceNet-ndBounces

the explanation of the wavelight at the beginning is just to tell that the light penetrates very shortly in the guide, just enough to eccitate the released fluorophore in order to be detected, since it needs to be eccitated by uv light to be detected, otherwise you could see all possible background light given by sopntaneous reaction or whatever

Nereus

I don't really understand 0:23. mind explaining?

jongerchua

can someone explain to me does the unincoporated fluoresecently tagged nucleotide cause noise when measuring the signal of the incorporated nucleotide?

abnormalphysics

Great Video. Im studying biotechnology and this helped me a lot in genomes.

raphaeltiziani

Nice video. Clear explanations and animations.

pablohoney

Could you help me explain what happen at the other end? Can you somehow fix two polymerase at the bottom? Do both forward and reverse synthesis happen at the same time? Thanks.

trucmai

Imagine you are an international student paying 5, 000 dollars for this course to learn in university and they are sent to off to watch an advertisement video from a tech company, a silent comedy film, no less.

yilongzhang

any one understand how excaly hesy choose or isolate a single dna fragment or template of desire ?

marwanmohamed

10^-21 liters is a single zeptoliter, not 20 zeptoliters.

robertlogan

Great Video. Im studying biotechnology and this helped me a lot in genomes.

raphaeltiziani

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