Polyclonal antibody

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This lecture explains about polyclonal antibody structure and the production of polyclonal antibodies. It also explains the use of polyclonal antibody in biological techniques like immunobloting.
Polyclonal antibody lecture – In this video lecture Suman Bhattacharjee shares information about the principles of Polyclonal antibody. It also explains the role of Polyclonal antibody. Watch this lecture to know more details about the Polyclonal antibody.

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U explain really great
I have 10 days for my enterence exam and with your videos i have completed so many things in less time and u explain in such a simple way tht it's really easy to remember

Thats_my_tale

you are really simplifier, and recently detected your Chanel accidentally, and it's now one of my first sources for referring when things go tough in biology . Thanks

tusiye

Polyclonal are those when serum contains different ab against single antigen for different epitope and each one is specific for one epitope. I think you might have told unconsciously that one ab recognise different epitope.

pritiswain

If you inject a specimen A (let's say a mouse) with a specific antigen, this host will maturate lymphatic B-cells into B-plasma cells (through the known pathway macrophages - dentritic cells - lymphatic T-cells - T-helper cells) that produce antibodies against it. These Ab can be extracted as a serum. Normally, an antigen has more than one epitope with which an antibody can interact. Therefore, the extracted serum/mixture would contain different antibodies against the antigen, (as a whole) so-called polyclonal antibodies. Still, each of the antibodies can only interact with one specific epitope expressed on said antigen.
Hope that helps/is at least accurate :D

michaelleischnig

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anyannoaxom

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Semsempharma

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Talentcreationndimagination

Love the way... You explained sir... Hats off Sir jee

rahilabbas

Specificity and affinity these are the unique words used in this video ❤️

bhargavkbh

Polyclonal antibody is actually collection of antibodies produced from different B cell lineages that's why they can interact with different epitopes of same antigen. Am I right?

spanditaroy

Thank you sir for such a informational vedio

jyoti

You have the general idea right but off when you get into specifics. For a start, there is no such thing as a polyclonal antibody. It would be synonymous to saying someone has multiple birth mothers! Impossible right?! Polyclonal is the mixture of antibodies from multiple cells before clone selection. Yes, the mixture will target different epitopes but on a micro level, each antibody can only be specific to a single epitope.

MegaMie