Enzymes: Extracting Biological Insight Using UniProt

UniProt is the world’s leading high-quality, comprehensive and freely accessible resource of protein sequence and functional information. Enhanced enzyme annotation in UniProtKB is based on Rhea, a comprehensive expert-curated database of biochemical reactions that uses the ChEBI ontology to describe reaction participants.

Starting with a biological question (I.e., Could you refine my list of enzymes to identify the ones with cysteine at the active and/or allosteric sites?), you will explore the different ways of answering it, using UniProt's advanced query, or SPARQL when the use of UniProt advanced queries is not applicable. Hints on how to extend queries to other biological contexts will also be provided.

This webinar introduces the enzyme-related data in UniProt and how to find it. The video was recorded live during the SIB course streamed on June 6, 2024.

Target audience: This webinar is for beginners interested in protein enzymes.

Speakers: Elisabeth Gasteiger and Jerven Bolleman, Swiss-Prot group

Links:

Chapters:
0:00 - Introduction
0:27 - Part I: What you need to know to make your UniProtKB queries meaningful
31:46 - Part II: SPARQL

[bamhre]

Great presentation, thanks!
How many of the ~5, 000 human enzymes have been targeted with clinical medicines? Is Lipinski's 'rule of five' still valid? He claimed there are ~500 enzymes considered "bottleneck" in biologically high impact nodules.