filmov
tv
#22976 Increased oxidative stress with age negatively impacts the urinary bladder urothelium
Показать описание
Increased oxidative stress with age negatively impacts the urinary bladder urothelium
de Rijk M1, Wolf-Johnston A2, Kullmann A2, Taiclet S2, Shiva S2, Birder L2
1. Department of Urology, School for Mental Health and Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, The Netherlands, 2. Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh PA 15261 USA
KEYWORDS: Animal Study, Basic Science, Biochemistry , Gerontology, Sensory Dysfunction
The prevalence of lower urinary tract symptoms (LUTS), characterized by problems regarding storage and/or voiding of urine, is known to drastically increase with age. The increased incidence of LUTS is often related to age-related changes in the urinary tract, surrounding structures (e.g. prostate) or the central or peripheral nervous system [1,2]. In an aging society LUTS are, therefore, highly relevant and increasingly important. When involuntary loss of urine occurs together with a decrease in mobility, as is often the case in aging, this can lead to a decline in self-reliance and is often one of the decisive factors in the decision for older adults to move to institutionalized care.
The inner surface of the bladder is covered with a mucosal membrane. This bladder mucosa consists of a multi-layered urothelium and underlying lamina propria. The urothelium fulfills crucial tasks in serving as a protective barrier protecting the underlying bladder tissue from the harsh chemical composition of urine, and exhibits signaling properties via release of mediators within the bladder wall that affect bladder functioning. The proper execution of these tasks is highly dependent on healthy functioning of urothelial cells [3].
The exact mechanisms by which aging related processes influence urothelial health and bladder functioning are not yet fully understood. In the current study we aimed to further elucidate aging related changes in urothelial and mucosal biochemistry. We investigated biomarkers for oxidative stress in mucosal tissue samples collected from young and aged rodents, and assessed cellular respiration, mitochondrial functioning and basal ROS in urothelial cell (UTC) cultures. We hypothesized that aging increases oxidative stress which in the long term may damage multiple components of the LUT system.
de Rijk M1, Wolf-Johnston A2, Kullmann A2, Taiclet S2, Shiva S2, Birder L2
1. Department of Urology, School for Mental Health and Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, The Netherlands, 2. Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh PA 15261 USA
KEYWORDS: Animal Study, Basic Science, Biochemistry , Gerontology, Sensory Dysfunction
The prevalence of lower urinary tract symptoms (LUTS), characterized by problems regarding storage and/or voiding of urine, is known to drastically increase with age. The increased incidence of LUTS is often related to age-related changes in the urinary tract, surrounding structures (e.g. prostate) or the central or peripheral nervous system [1,2]. In an aging society LUTS are, therefore, highly relevant and increasingly important. When involuntary loss of urine occurs together with a decrease in mobility, as is often the case in aging, this can lead to a decline in self-reliance and is often one of the decisive factors in the decision for older adults to move to institutionalized care.
The inner surface of the bladder is covered with a mucosal membrane. This bladder mucosa consists of a multi-layered urothelium and underlying lamina propria. The urothelium fulfills crucial tasks in serving as a protective barrier protecting the underlying bladder tissue from the harsh chemical composition of urine, and exhibits signaling properties via release of mediators within the bladder wall that affect bladder functioning. The proper execution of these tasks is highly dependent on healthy functioning of urothelial cells [3].
The exact mechanisms by which aging related processes influence urothelial health and bladder functioning are not yet fully understood. In the current study we aimed to further elucidate aging related changes in urothelial and mucosal biochemistry. We investigated biomarkers for oxidative stress in mucosal tissue samples collected from young and aged rodents, and assessed cellular respiration, mitochondrial functioning and basal ROS in urothelial cell (UTC) cultures. We hypothesized that aging increases oxidative stress which in the long term may damage multiple components of the LUT system.
0:05:51
0:01:11
0:14:31