Preparation of frozen tissue sections (Cryotomy)

While the sectioning of formalin-fixed, paraffin-embedded tissue is the norm in most histology laboratories, sectioning of freshly-frozen tissue supports specialised tests in a fraction of the time. This video discussed these concepts while demonstrating the operation of a cryostat.

[ashpingv]

As an anatomic pathologist. Much appreciation for this video! Hope you can sample other tissue such as lymph node.

[joelscott]

Nice vid always wondered what that anti roll plate was for as ones at work dont have the glass piece

[rezamoosavi]

Your videos are very useful, I greatly appreciate them!

[aamirrashidkhan]

Thanks Damien and Chris, wonderful job!! very useful video for early learners!

[nabarcheto]

Thank you so much! So well and clearly explained! Really, thank you!

[yijingwang]

Hello, I wonder will you dry out your slides at Room temperature after cutting?

[linhmaithuy]

Have a great day sir. I have a question: with spleen, lymph node specimens, should the section after being embedded in paraffin be cut thinner or thicker than usual ? And why we shoud do that ? Thanks for answering me !

[PR-nfot]

Hi. Thanks for this video. I am working with spheroids from cell culture . What is your suggestion for thickness? Thanks

[lotharmayring]

tissu can be fixed before frozen or within the freezing process appling crygel with glutaraldeyd

[T.J.]

How is the frozen section held across the blade? like in paraffin sectioning you have the wax covering the entire specimen, giving it a rectangle shape, since no such embedding is done in cryotomy, do you just place the whole thing in the holder?

[captaincrunch]

does tissue sectioning for ovary also uses cryostat?

[janvimore]

Thank you Sir.... It's really helpful

[jmslrnz]

What is the embedding medium for frozen sections called?

[coastalplayer]

ASCP 30 years fromTexas, very nice technique