Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows

Presented By:
Tia Hexom, PhD - Field Applications Scientist, Cell Therapy, Thermo Fisher Scientific

Speaker Biography:
Doctorate in Cell and Molecular Biology from Tulane University. Studied chronic variable stress and its effects on dendritic morphology and synaptic plasticity. From there, I did an NIH fellowship at UCSD looking at Alzheimer's disease and stress hormone signaling in human hippocampus and human NSCs (neural stem cells) derived from iPSCs. Spent the last 5 years working in cell therapy with regard to primary cells, 510k medical devices, and cell separation technologies. My favorite things to do in my free time are travel the worlds and see it's many museums, and go hiking or to the beach with my dog.

Webinar:
Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows

Webinar Abstract:
Induced pluripotent stem cells (iPSC) intended for translational applications need to be of high quality; free of reprogramming transgenes, clear of adventitious agents, genetically stable, and functionally pluripotent. Generation of such cells requires consistent reprogramming, defined culture reagents and comprehensive characterization. CytoTune Sendai virus reprogramming enables consistent and robust reprogramming of diverse somatic cell types. Here we report the first off-the-shelf (CTS) Sendai virus reprogramming kit and a xeno-free workflow from fibroblast and blood cells designed for clinical and translational use. iPSCs were generated under these conditions from fibroblasts, CD34+ and T cells. To further streamline characterization, three molecular methods PluriTest, ScoreCard, and KaryoStat were used to confirm pluripotency, trilineage differentiation potential and genome integrity of the iPS cells.

Learning Objectives:
-The importance of consistent reprogramming, defined culture reagents and comprehensive characterization for generation of iPSC cells
-Details for the first off-the-shelf (CTS) Sendai virus reprogramming kit
-Review of three molecular methods PluriTest, ScoreCard, and KaryoStat and how they were used to confirm pluripotency

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