DNA analysis | NMDCAT 2021

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About This Video ........................

In This Video Lecture You Will Learn Basic Concept Of DNA Analysis
The entire genome of an individual can be subjected to DNA inger printing, a process
described in Fig. 23.6. The genome is treated with restriction enzymes, which results
in a unique collection of diferent sized fragments. Therefore, restriction fragment
length polymorphism (RFLPs) exists between individuals. During a process called gel
electrophoresis, the fragments can be separated according to their lengths (molecular
they appear as a smear. However, the use of probes for genetic markers produces a
weight or size), and the result is a number of bands that are so close together that
distinctive pattern that can be recorded on X-ray ilm.
The DNA from a single sperm enough to identify a suspected rapist. Since DNA
PCR ampliication and analysis can be used (1) to diagnose viral infections, genetic
is inherited, its inger print resembles that of one’s parents. DNA inger printing
and F2
successfully identiied the remains of a teenager who had been murdered eight years
before because the skeletal DNA was similar to that of the parent’s DNA.
of the victim.Therefore suspect 1 is the culprit. The suspect 2 and 3 are not.
disorders, and cancer (2) in forensic laboratories to identify criminals; and (3) to
The arrows on left side show common bands between mother and child while those on
DNA from both of his parents. Obviously F1
sequence DNA taken from a 76,000 years old mummiied human braiti and from a 17
Fig. 23.7 (b) shows a case of disputed parenthood. Two persons F1
determine the evolutionary history’ of human population. It has been possible to
is not the real father.
his parents. Arrows indicate that some bands in him are like his father, some like his
from murderer’s skin and hair, and from three suspects blood. Can you compare them
cells from underneath her nails. They prepared DNA inger prints from blood of victim,
for speciic DNA sequence and tell who is in guilty and who is not? The suspect 1 has
claim to be the
father of child C, whose mother’s inger print is given under M. The child has received
Fig. 23.7(c) shows DNA inger prints which have been presented as forensic evidence. A
parents.
child’s inger print with that of his parents. The child has received DNA from both of
mother. Some bands are. however, unique to him, which do not match with any of the
criminal on a deserted place assaulted a woman. She scratched his face in her defence
right show common bands between the father and the child.
In Fig. 23.7 are given some DNA inger prints. The igure 23.7 (a) shows comparison of
but he murdered her and ran away. Forensic scientist recovered murder’s hair and skin
inger prints, which is similar to linger print from skin cells taken from underneath nails
to 20 million years old plant fossil following PCR ampliication.

About This Channel.....................

I make these videos cause I love to draw and connect the complexity of science and medicine into art. I'm not saying. I'm 100% correct in all my videos, but I do try to obtain the information from credible sources.
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