Plate Reading - Urine I

Reading two urine cultures.

This video is for instructional uses only and is not to be used for actual medical laboratory testing.

[herbertharvey]

I get it now. You are a good teacher. You made me understand by this video. Thank you sooo much!!!

[meryemlahbara]

Thank you very very much ... I have a crappy tutor in my internship & your videos are help ..

[rnbrineg]

This is great, a good quick review for my micro class!

[kiannasksksgwongsksk]

@Patrick Tracy Hii! May I ask how to count the colony if we did not use any dilution? That's why we cannot use the CFU/ml, we are having a hard time to compute for it. Here's what we did. We get the sample from the keyboards using cotton swab then we directly streak it to MSA.

[mirzasikandar]

7:24 but these are LF colonies why we do oxidase test on these....we only do on NLF plz clear your point

[onegod]

great video sir... please do more reading cultures videos

[nwachukwupeace]

Please can you show other tests and how you inoculated and all for better understanding

[paulhernandez]

My culture test says "No microorganism seen" in the gram stain. However, the C/S Bact PF identified the presence of coagulase negative staphylococcus with "growth isolated after 24 hours from bottle." The bacterial count is N/A and there is "no growth on primary isolation for colony count." Can somebody explain?

[asdfz]

What if a symptomatic patient's urine culture does not does not fall within the range to justify an "infection"? Are there uti-causing bacteria that will not grow in a standard urine culture? Are there other tests out there that can detect infectious organisms in urine? Thank you!!

[staisi]

hello, thank you so much for your videos its so helpful .
I have a question, why do you count on blood agar, and not on macconkey agar or others agar !?
Thank you .

[prm]

Really appreciate your video! I have a question though. What is the impact if in doing a Gram stain, the decolourizer is not rinsed with water before adding safranin, I.e., decolourizer is just drained from the slide?

[muhammadtaimurkhan]

Can we culture urine directly like csf and sputum samples before inoculating on the plates first.
Is it really gram stained at all?

[scientistbug]

Thanks Sir Patrick.. I needed your videos so much... 🙌🏻

[nwachukwupeace]

What about the biochemical tests.... you didn't show how to do it

[talalkhayata]

Why do u need a gran test if C&A is already telling us that its Gram +ve?

[kiannasksksgwongsksk]

Please help us, we really need your help. Thank you in advance.

[fadiawaheed]

Can you please recommend me book which you are following

[mohammadabbassafi]

how to report 100 colonies as 100, 000 CFU/ml ? explains sir

[nananti]

Please something you need to speed up a little bit, the talking is something too much, pls go straight to the point.

[eli-kwfmjc-doctrine]

ua gn
ua gp
ua many

setup:
sba: streak for count: >100, 000 cfu/ml
mmac/cna: no growth on cna; lactose pos on Mac

Mac: selective for gno: differential for lactose fermentation
/cna: cna (selective differ for gpo and yeasts) differential for hemolysis
calibrated loop: 1/100 or 1/1000 ml loop
report: >100, 000 cfu

how to work it up: indwelling catheter, suprabuppic aspirate;
oxidase test: read of...
oxidase neg
Media:

report: is it contamination, infection or....
>100, 000 cfu/ml of probable ecoli
id/susp to follow

Ecoli: no 1 infection in women.
How to QC plates:

ua 2nd sample
Mac
cna selective for gpo and yeasts

sba: count >100, 000 cfu/ml work it up
if gpo that are pathogens in ua: staph saprophyictus, yeast, enteropathogens
see feet in this plate. work it up as yeast.
gs: and work it up; f? yesasts
Preliminary report: >100, 000 yeast; id to follow