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The Comet Assay - A Versatile and Sensitive Technique for the Assessment of DNA Damage and Repair
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The Comet Assay is a powerful tool for the assessment of DNA integrity with applications as diverse as cancer research, safety testing of pharmaceuticals and chemicals, environmental and occupational studies, dietary and even fertility research.
As the assay can be adapted to virtually any cell-type from almost any organism, study designs can be created to characterize DNA damage and repair as well as DNA integrity and applied to both the impact of treatments and exposures as well as to endogenous factors. In this webinar two well-known scientists in this field describe recent research applications the comet assay.
As the assay can be adapted to virtually any cell-type from almost any organism, study designs can be created to characterize DNA damage and repair as well as DNA integrity and applied to both the impact of treatments and exposures as well as to endogenous factors. In this webinar two well-known scientists in this field describe recent research applications the comet assay.
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The Comet Assay - A Versatile and Sensitive Technique for the Assessment of DNA Damage and Repair
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STEP 1: Preparation of substrate cells // Comet-based in vitro DNA repair assay
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Step 4: Comet visualisation & analysis // Measuring DNA damage using the comet assay
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Step 2B: Lesion specific enzyme incubation // Measuring DNA damage using the comet assay
Step 2: Embedding cells in agarose // Measuring DNA damage using the comet assay
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